[mira_talk] Re: Editing/ cleanup of MIRA assembly with gap5

  • From: Rameez Mj <rameez03online@xxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Wed, 16 Dec 2015 23:22:45 +0530

Make sure the "databasename .g5x" (which is created along with database.g5d
while using tg_index on .caf file) is also present in the same folder.

On 14 December 2015 at 13:50, Chitra P <pattabiraman.chitra@xxxxxxxxx>
wrote:

Hi,

Thank you for the reply.I just tried using the CAF file as the input for
tg_index however I am still unable edit (or see the effect of my editing?).
Is there some other file I need to load into gap5 other than the .g5d ?

Thanks,

Chitra

On Mon, Dec 14, 2015 at 1:29 PM, Andrej Benjak <abenjak@xxxxxxxxx> wrote:

I used to use tg_index on the original CAF file and have never had
problems. Could you try?

cheers,
Andrej


On 12/13/2015 06:58 AM, Chitra P wrote:

Hi,

I know this is slightly off-topic for the MIRA list, but I would be
grateful for any help with this.

I want to edit a MIRA assembly with gap5 ( I have installed
staden-2.0.0b9.i686 on a Ubuntu 14.04). At present, I am able to load but
unable to edit the sequences.

This is what I did :
I converted the MIRA maf and fasta files to sam

then used tg_index to convert it to a gap5 database

I am able to view the sequences but unable to edit the sequence. In my
terminal get the following error message when I try editing

*'D8YB9:08803:07440': couldn't open*


Thanks,

PC




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