[mira_talk] Assembly using Illumina Paired-end reads from SRA
- From: Chayan Roy <chayan.roy93@xxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Tue, 18 Feb 2014 00:56:25 +0530
Hii
I have downloaded an Illumina Pair-end data set from SRA and used
fastq-dump from SRA tool-kit to generate the fastq file for that..well
everything works fine till here..now i wanted to reassemble it with
MIRA..but from SRA i got only one file for pair end reads, as they
mentioned that it will be a joined (may be by cat ???) file...now i am not
sure how to a single file for a PE assembly with MIRA..somewhere i found
that they mention it to treat it as a unpaired one but then should i use
readgroup = unpaired/PE... in both cases MIRA is complaining...little
confuse..any one has any experiences with this???
thanks for any help
best ...
--
*CHAYAN ROY*
Other related posts:
