Hi,
after years of absence I am now involved in project where I think MIRA is
the perfect option:
I have a pool of amplified antibody sequences (~600bp, pe100 illumina
data). All these sequences are extremely similar by nature.
I want to assemble these fragments, so that every potential ab sequence
builds its own contig, avoiding (or trying to avoid) any IUPAC in the final
consensus.
Final contig set should represent the ab input pool / diversity.
Singlets will be removed in final dataset ..
Comments/parameter recommendations how to accomplish this are highly
appreciated :-)
I am about to use 4.9.6 on linux.
best,
Sven
