[mira_talk] Re: About ends clipping
- From: "Yi Zheng" <yz357@xxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Tue, 28 Apr 2009 10:51:01 -0400 (EDT)
Hi Bastien,
Thank you for you reply.
We are currently using mira to assemble cDNA (EST) sequences which should
be low coverage of the transcriptome.
If I switch off the -CL:pec as you recommended, we found that quite a few
sequences not assembled together only differ in a short
stretch of the ends (which are usually in relatively low quality). If I
switch on, then the information of a lot of alternative
splicing will be lost.
Hope you can add this function to mira and thank you so much.
Yi
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