[mti] Unusual regular PCR bands in non targets
- From: "Peters, Kristian \(PIRSA - SARDI\)" <peters.kristian@xxxxxxxxxxxxxxxx>
- To: <mti@xxxxxxxxxxxxx>
- Date: Tue, 27 Mar 2007 13:30:42 +0930
Good work Simon organising this discussion group. I am sorry I have no answers
as yet. I also have a question. I have designed a species specific primer that
targets mtDNA of a marine species used in a feeding trial. It works fine
amplifying the target sequence, but non targets produce unusual light regular
banding (much like bp ladder but finer) throughout the entirety when run on a
2.0% gel. I don't think it is a contaminant, but something else in the
reaction. We have changed over reagents to determine the problem with no
success. Has anyone experienced this in their PCR products?
Cheers
Kristian Peters
SARDI Aquatic Sciences
0428993524
________________________________
From: mti-bounce@xxxxxxxxxxxxx on behalf of simon jarman
Sent: Fri 23/03/2007 11:17 AM
To: mti@xxxxxxxxxxxxx
Subject: [mti] pseudogenes and chimaeras
Hello,
Thank you all for subscribing. There are now more than 30 people on
the list, which is a good starting point for a discussion group.
I have a question. Have any of you identified pseudogenes or chimaeric
sequences when using universal-type primers to amplify animal mitochondrial
genes from dietary samples?
--
Dr Simon Jarman
simon.jarman@xxxxxxxxx
(+61)0408580923
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