[mira_talk] Re: two different contig with same sequence
- From: Bastien Chevreux <bach@xxxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Thu, 3 Dec 2009 20:51:41 +0100
On Donnerstag 03 Dezember 2009 Shai Uliel wrote:
> Can you give the explanation when MIRA write SNP (W,R....) and when
> MIRA create two contigs ??????
Did your keyboard got stuck on the question mark? :-)
The simple explanation: if MIRA feels certian enough, it'll creates two
contigs. If not, it looks whether there's enough evidence to call a single
base as consensus (ACGT). If two or more bases have approximately the same
probability to be a real base, it calls a IUPAC code base (W,R,...).
Regards,
Bastien
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