Well, unfortunately there is no much information. It is one of those "orphan" plants. But the polymorphisms among copies of the same gene can be as many as 3-5 SNPs per gene. So around 1-3 SNPs per 1000 bp. Thanks Juan From: mira_talk-bounce@xxxxxxxxxxxxx [mailto:mira_talk-bounce@xxxxxxxxxxxxx] On Behalf Of Bastien Chevreux Sent: Sunday, February 23, 2014 8:07 AM To: mira_talk@xxxxxxxxxxxxx Subject: [mira_talk] Re: tweaking Manifest for polyploid genome On 22 Feb 2014, at 20:55 , Gutierrez, Juan <Juan.Gutierrez@xxxxxxxxxxxx<mailto:Juan.Gutierrez@xxxxxxxxxxxx>> wrote: I am trying to do RNA-seq de novo on a polyploid (hexaploid) genome using a combination of 454 and illumina 100bp paired ended reads. The three copies of each gene are highly similar to one another. Care to expand a bit more on the "highly similar" criterion? Is it one diff per 100bp, per 1000bp, per 10000bp, ...? B. This electronic message contains information generated by the USDA solely for the intended recipients. Any unauthorized interception of this message or the use or disclosure of the information it contains may violate the law and subject the violator to civil or criminal penalties. If you believe you have received this message in error, please notify the sender and delete the email immediately.