[mira_talk] Re: tweaking Manifest for polyploid genome

  • From: "Gutierrez, Juan" <Juan.Gutierrez@xxxxxxxxxxxx>
  • To: "mira_talk@xxxxxxxxxxxxx" <mira_talk@xxxxxxxxxxxxx>
  • Date: Sun, 23 Feb 2014 21:56:12 +0000

Well, unfortunately there is no much information. It is one of those "orphan" 
plants. But the polymorphisms among copies of the same gene can be as many as 
3-5 SNPs per gene. So around 1-3 SNPs per 1000 bp.

Thanks
Juan

From: mira_talk-bounce@xxxxxxxxxxxxx [mailto:mira_talk-bounce@xxxxxxxxxxxxx] On 
Behalf Of Bastien Chevreux
Sent: Sunday, February 23, 2014 8:07 AM
To: mira_talk@xxxxxxxxxxxxx
Subject: [mira_talk] Re: tweaking Manifest for polyploid genome

On 22 Feb 2014, at 20:55 , Gutierrez, Juan 
<Juan.Gutierrez@xxxxxxxxxxxx<mailto:Juan.Gutierrez@xxxxxxxxxxxx>> wrote:
I am trying to do RNA-seq de novo on a polyploid (hexaploid) genome using a 
combination of 454 and illumina 100bp paired ended reads. The three copies of 
each gene are highly similar to one  another.

Care to expand a bit more on the "highly similar" criterion? Is it one diff per 
100bp, per 1000bp, per 10000bp, ...?

B.






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