[mira_talk] Re: combining unpaired and paired-end assembly (454)
- From: "Ondov, Brian D" <ondovb@xxxxxxxxx>
- To: <mira_talk@xxxxxxxxxxxxx>
- Date: Mon, 29 Mar 2010 09:58:04 -0400
Nevermind...I didn't see the "-a" flag in the second extract in the
walkthrough (which causes append instead of rewrite).
From: mira_talk-bounce@xxxxxxxxxxxxx
[mailto:mira_talk-bounce@xxxxxxxxxxxxx] On Behalf Of Ondov, Brian D
Sent: Thursday, March 25, 2010 2:57 PM
To: mira_talk@xxxxxxxxxxxxx
Subject: [mira_talk] combining unpaired and paired-end assembly (454)
There is an example of how to do this in the 454 docs, but it looks like
the commands will just clobber the extracted single-read files
(bcet.fasta, etc.) when extracting the paired-end files. Because of the
input file naming scheme, it seems like there can only be one data set
for each platform. Should I be doing one sff_extract with all the data
at once (and just letting it try to find the non-existent linker in the
unpaired data)?
If it matters, I'm referring back to 3.0.0 docs, since the doc/docs/
folder seems to be missing in later releases...
-Brian
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