[mira_talk] Re: Strange mapping results
- From: Lionel Guy <guy.lionel@xxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Mon, 5 Jul 2010 10:41:27 +0200
On 2 Jul 2010, at 19:43 , Bastien Chevreux wrote:
To be honest, I don't have a really good solution for that (and I
tried quite
a lot). Fortunately, 36mers are a thing of the past, 75 and 100mers
take over
and there the problem is much less pronounced.
But the question is not only in the alignment, IMHO, but also in the
way MIRA calculates the consensus: in that very case, I have 8 solexa
reads saying "A", 30-40 454 reads and a good 50-100 solexa reads that
say "*", and the consensus is called as "A", which sounds really weird
to me. Could it be that these few solexa reads that end by one base
screw the majority rule applied by mira?
The STMS tag reads: "Solved vote: / sxa (1) A 41 is in ", whereas
there are not 41 solexa reads that say A, only 8...
Lionel
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