> On February 17, 2014 at 4:05 PM Peter Cock <p.j.a.cock@xxxxxxxxxxxxxx> wrote: > I think there is a problem in the MIRA 4.0 SAM output with FLAG bit > fields 0x10 and 0x20 (partner read's strand) sometimes being wrong, > giving bad FLAG value pairs like 83 and 131, or 99 and 179. IcanhazMAFplease? It's easier to track down problems on a live data set. Related: how did you find those problems? I should take up a similar toolset to spot potential problems earlier. B.