Hi Bastien, > On Thursday 13 January 2011 12:43:03 Jorge.DUARTE@xxxxxxxxxxxx wrote: > > Problem is, it is difficult to tune the parameters with such long assembly > > times... (1st pass was completed in one week for default parameters, and i > > had to kill the process because of a shut down of our server for > > maintainance) > > I am a bit surprised that 450k reads need 1 week for a first pass. May I have > the output log to see what takes so long? I'd guess that there are acouple of > very highly expressed transcripts. I am not assembling 450000 reads but 8 times 4500000, so arround 3.6M 454 titanium reads when puting all strains together. I don't have access to the log file as my server is down at the moment, but i'll send it to you as soon as i can. Do you want the entire file, or let's say the 10000 first lines would be enough ? thanks jorge.