[mira_talk] Question about merging results from multiple mira runs
- From: Robert Bruccoleri <bruc@xxxxxxxxxxxxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Tue, 23 Mar 2010 22:03:34 -0400
Dear Colleagues,
I am working on a project to assemble a bacterial genome using a
combination of Solexa and SOLiD technologies. The data is too large for
our limited memory computers to handle, so I'm trying divide and conquer.
I've split the reads into smaller pieces that can all be assembled
by mira, and now I would like to combine the results. One possibility
would be to combine the contigs and debris sequences and qualities score
into one pair of files, and run mira on those, but that would preclude
any reassemblies based on the new information. Another possibility would
be assemble one subset, and map each additional subset onto it. A third
possibility would be to combine two assemblies together, but I didn't
see any documentation that such an operation was possible.
Has anyone tried any of these approaches? If so, what successes or
failures did you have?
Thanks. --Bob
begin:vcard
fn:Robert Bruccoleri
n:Bruccoleri;Robert
org:Audacious Energy, LLC and Congenomics, LLC
adr:;;60 Gates Farm Road;Glastonbury;CT;06033;USA
email;internet:bruc@xxxxxxx
title:President
tel;home:860 633 6655
tel;cell:609 902 8419
version:2.1
end:vcard
Other related posts:
