On Jul 26, 2011, at 0:35 , Diogo Santos wrote: > I think you hit the problem. My organism is a cross between two species of > plants and thus may be a great allelic diversity. But this explains the big > difference between MIRA and Newbler 2.5? Is it possible to rely on the > results of MIRA or in this case it is preferable to change parameters? > Sorry for the insistence ... Well, I trust MIRA most of the time ... if I don't catch it doing some foolish things that is. In your case, I'd take a couple of well know genes and look in a finishing program what MIRA did and what Newbler did. You need to get a feeling for how the programs behave. “†rust“ is probably misplaced here ... B.