Hi all, I ran miraSearchESTSNPs on wheat 454 Titanium EST dataset which is only for 1 strain. In step 2, I considered the union of pristine transcripts in "default' and "remain" folders as my finally assembled transcripts (conding exisitng proteins). In step 3, I found the consensus sequences may be assembled from step-2 "default" and "remain" pristine transcripts. Because of the remain transcripts, the detected SNPs in these consensus sequences have 'SROc' / 'SIOc' types. Because the input EST dataset is in 1 strain, could I consider the 'SROc' and 'SIOc' SNPs the same as 'SAOc', and used the three types in analysis. Thank you. Xiaomei