[mira_talk] Re: FW: 454 assembly
- From: Veljo Kisand <vkisand@xxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Thu, 22 Jul 2010 11:02:47 +0300
Shabhonam Caim (TGAC) wrote:
*Thanks thomas for your reply but still I am getting error:*
I have used the following command and provided the qual file as well :
mira --project=test --job=denovo,genome,draft 454_SETTINGS
-FN:fai=2.GAC.454Reads.fna -FN:fqui=2.GAC.454Reads.qual
i think one problem is there is no no traceinfo
i use an old way to feed date in kind a standard file name format -
which will be created on extract / for 454 standard clipping use switch -c/
sff_extract -s test_in.454.fasta -q test_in.454.fasta.qual -x
test_traceinfo_in.454.xml 2.GAC.454Reads.sff
and the run mira
mira --project=test --job=denovo,genome,draft,454
veljo
ps: i must admit never used -FN switches
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