On 08 Oct 2014, at 14:53 , Bobby Paul <bobbypaul29@xxxxxxxxx> wrote: > >In MIRA, each read is aligned only once. > If so it means reads from tandem repeat regions as well as paralogue gene > regions will make single conting right? You’re talking about de-novo here, right? Maybe yes, maybe not: if the gene copies are different enough or are short enough to be crossed by single reads or paired reads, they will be in different contigs for sure. > The fourth query was is there any chance to align single read in multiple > location. If this is about mapping: every read is mapped exactly once into the genome. If several possibilities exist where to map, the reads are evenly distributed. B. -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html