G'day all, I am in the middle of an assembly of a 5 megabase bacterial pathogen, using about 40-fold 454 and 70-fold illumina (paired end) reads. It's something we have done 60 or 70 times. As I was watching the output come scrolling by, a couple of hours into the assembly, I noticed the following error message: WARNING: contig did not put refid into affected reads. Please file a bug report to https://sourceforge.net/apps/trac/mira-assembler/ and please send a mail to mira_talk@xxxxxxxxxxxxx current af size: 4930 a WARNING: contig did not put refid into affected reads. Please file a bug report to https://sourceforge.net/apps/trac/mira-assembler/ and please send a mail to mira_talk@xxxxxxxxxxxxx current af size: 5090 aapaaaast+aasppppatspppppaaaa+aap+aapp 1141313 pft 3688 / 603 [1861047] ppaaatas+taappaa++ppptapaapaapapppppp+a WARNING: contig did not put refid into affected reads. Please file a bug report to https://sourceforge.net/apps/trac/mira-assembler/ and please send a mail to mira_talk@xxxxxxxxxxxxx current af size: 4526 a+a+aaatsaasapapappaa 1141313 pft 3609 / 649 [1861053] aasat+pappaaapappa WARNING: contig did not put refid into affected reads. Please file a bug report to https://sourceforge.net/apps/trac/mira-assembler/ and please send a mail to mira_talk@xxxxxxxxxxxxx current af size: 4443 aaaaaasaapaaa++ WARNING: contig did not put refid into affected reads. Please file a bug report to https://sourceforge.net/apps/trac/mira-assembler/ and please send a mail to mira_talk@xxxxxxxxxxxxx current af size: 4178 a+apps+aata+aasa+ttaasa+p+s 1141313 pft 1117 / 684 [1861062] atppaaaaspa+pp++aap+ WARNING: contig did not put refid into affected reads. Please file a bug report to https://sourceforge.net/apps/trac/mira-assembler/ and please send a mail to mira_talk@xxxxxxxxxxxxx current af size: 4731 aaaassaaassaaapaaassssssssssssasstss+s+s 1141313 pft 293082 / 383 [1861068] asssssssssstsssssssssstsssssssasaasss+asasasasts+ssassa+sass 1141315 pft 2 / 209 [1861071] s+tasss+sasssasaa+sssssassaastssassas++satsssssssstss+ssssss 1141315 pft 2 / 191 [1861077] aaatsssssssaasastasssssaatssssassssass+sssatsassatsasaassasa 1141315 pft 2 / 197 [1861078] sastttats+ttssstaaaaaaasa+sssssssssssssssass+sssssssapa+aaaa 1141316 pft 12 / 202 [1861082] aaaaaaaaaaaaaasttaaaas WARNING: contig did not put refid into affected reads. Please file a bug report to https://sourceforge.net/apps/trac/mira-assembler/ and please send a mail to mira_talk@xxxxxxxxxxxxx --- Just tell me what I need to do to help you, and I will send the appropriate data. John