[mira_talk] Bug report mira verison 3.9.9

  • From: John Nash <john.he.nash@xxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Thu, 10 Jan 2013 15:03:16 -0500

G'day all,

I am in the middle of an assembly of a 5 megabase bacterial pathogen, using 
about 40-fold 454 and 70-fold illumina (paired end) reads.  It's something we 
have done 60 or 70 times.

As I was watching the output come scrolling by, a couple of hours into the 
assembly, I noticed the following error message:

WARNING: contig did not put refid into affected reads. Please file a bug report 
to
        https://sourceforge.net/apps/trac/mira-assembler/
and please send a mail to
        mira_talk@xxxxxxxxxxxxx
current af size: 4930
a
WARNING: contig did not put refid into affected reads. Please file a bug report 
to
        https://sourceforge.net/apps/trac/mira-assembler/
and please send a mail to
        mira_talk@xxxxxxxxxxxxx
current af size: 5090
aapaaaast+aasppppatspppppaaaa+aap+aapp   1141313        pft     3688 / 603
[1861047]       ppaaatas+taappaa++ppptapaapaapapppppp+a
WARNING: contig did not put refid into affected reads. Please file a bug report 
to
        https://sourceforge.net/apps/trac/mira-assembler/
and please send a mail to
        mira_talk@xxxxxxxxxxxxx
current af size: 4526
a+a+aaatsaasapapappaa   1141313 pft     3609 / 649
[1861053]       aasat+pappaaapappa
WARNING: contig did not put refid into affected reads. Please file a bug report 
to
        https://sourceforge.net/apps/trac/mira-assembler/
and please send a mail to
        mira_talk@xxxxxxxxxxxxx
current af size: 4443
aaaaaasaapaaa++
WARNING: contig did not put refid into affected reads. Please file a bug report 
to
        https://sourceforge.net/apps/trac/mira-assembler/
and please send a mail to
        mira_talk@xxxxxxxxxxxxx
current af size: 4178
a+apps+aata+aasa+ttaasa+p+s   1141313   pft     1117 / 684
[1861062]       atppaaaaspa+pp++aap+
WARNING: contig did not put refid into affected reads. Please file a bug report 
to
        https://sourceforge.net/apps/trac/mira-assembler/
and please send a mail to
        mira_talk@xxxxxxxxxxxxx
current af size: 4731
aaaassaaassaaapaaassssssssssssasstss+s+s   1141313      pft     293082 / 383
[1861068]       asssssssssstsssssssssstsssssssasaasss+asasasasts+ssassa+sass   
1141315  pft     2 /
209
[1861071]       s+tasss+sasssasaa+sssssassaastssassas++satsssssssstss+ssssss   
1141315  pft     2 /
191
[1861077]       aaatsssssssaasastasssssaatssssassssass+sssatsassatsasaassasa   
1141315  pft     2 /
197
[1861078]       sastttats+ttssstaaaaaaasa+sssssssssssssssass+sssssssapa+aaaa   
1141316  pft     12 
/ 202
[1861082]       aaaaaaaaaaaaaasttaaaas
WARNING: contig did not put refid into affected reads. Please file a bug report 
to
        https://sourceforge.net/apps/trac/mira-assembler/
and please send a mail to
        mira_talk@xxxxxxxxxxxxx

---

Just tell me what I need to do to help you, and I will send the appropriate 
data.

John


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