[mira_talk] Re: Assembly after vector screening

  • From: Nestor Zaburannyi <nestor@xxxxxxxxxxxxx>
  • To: Bastien Chevreux <mira_talk@xxxxxxxxxxxxx>
  • Date: Wed, 31 Aug 2011 14:04:30 +0200

Dear Bastien,

Version 3.4 final. Bacteria. 146  bp. Average coverage is 37. My vector is in my
vector.fasta,  in  fasta  format. After the assembly i can not see any
blast  hits  for  vector  in  contigs,  though  i  did  without  msvs*
parameters = 1000.

> On Aug 31, 2011, at 12:33 , Nestor Zaburannyi wrote:
>> After the assembly of my organism that was sequenced from pool of cosmids, i
>> have thousands of UIPACs and repeats. Can anyone recommend some adjustments
>> to make this assembly better?

> Version of MIRA? What kind of organism? Length of reads, average
> coverage? What's the content of your "vector.fasta"?

> The number of "SRMc" is somewhat disconcerting ...

> B.

Sincerely yours
Nestor


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