Dear Bastien, Version 3.4 final. Bacteria. 146 bp. Average coverage is 37. My vector is in my vector.fasta, in fasta format. After the assembly i can not see any blast hits for vector in contigs, though i did without msvs* parameters = 1000. > On Aug 31, 2011, at 12:33 , Nestor Zaburannyi wrote: >> After the assembly of my organism that was sequenced from pool of cosmids, i >> have thousands of UIPACs and repeats. Can anyone recommend some adjustments >> to make this assembly better? > Version of MIRA? What kind of organism? Length of reads, average > coverage? What's the content of your "vector.fasta"? > The number of "SRMc" is somewhat disconcerting ... > B. Sincerely yours Nestor -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html