[mira_talk] Adding in reads from PCR sequencing

From: John Nash <john.he.nash@xxxxxxxxx>
To: mira_talk@xxxxxxxxxxxxx
Date: Mon, 18 Apr 2011 11:34:11 -0400

Good morning all,

I am requesting some wisdom from the mira gurus.

After assembling a genome using mira, I have PCR-sequenced regions that 
indicated that they needed further examination.

Typically, does one re-assemble with the new sequences or is it preferable to 
manually add the sequences using gap4, or is there a better approach?

Thanks
John


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- [mira_talk] Re: Adding in reads from PCR sequencing
  - From: Davide Sassera

[mira_talk] Adding in reads from PCR sequencing

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