[ SHOWGSD-L ] Re: reproductive vet question
- From: "Peggy" <pmick12@xxxxxxxxxxx>
- To: <caretti1@xxxxxxxxxxx>
- Date: Fri, 26 Oct 2007 11:17:46 -0400
Regarding the test tube thingy...
most labs will make a notation on the result sheet if it's sent
in a serum separator tube (grey and red) that the result will be off. The
SST needs more tech time at your vets; the blood has to clot (about 15
minutes), then spin the blood down, decant and place the serum in yet
another plain red top tube before you send it in. The SST can sit on a
counter for a while, then just spin it down- the gel rises and will sit
between the red blood cells and the serum. But the gel can intereact with
the serum and alter some test results.
The labs may have some differences in protocols depending on their
machinery- but all protocols are in the books sent to the vet and the most
common ones are printed on the lab request sheets.
From Antech in 2004- but this was known before then:
Hormone Assays on Samples from Serum Separator Tubes
Becton Dickinson recently alerted us to the spuriously elevated
hormone tests results obtained when samples collected in their serum
separator tubes (SST) are measured by chemiluminescence technology
(Immulite®). Antech Diagnostics uses primarily radioimmunoassays and has
recently evaluated the effect of sample collection into SST on progesterone,
thyroid, and cortisol concentrations, when measured by radioimmunoassay
(RIA).
Progesterone concentrations measured by RIA may be falsely decreased
in samples collected using SST. The sample of choice for progesterone
concentrations is separated serum (from a sample collected originally into a
plain red top tube).
There is no effect of sample collection into SST on thyroid or
cortisol concentrations, when measured by RIA. SST or separated serum
samples are appropriate for cortisol and thyroid testing by RIA.
So much for that.........
Peggy
>
>> Repro questions.....ah, a topic I will participate in!
>>
>> .....One thing I learned at the education seminar at the Nat'l,
>> and maybe
>> everyone else already knows it.....Progesterone levels (actually
>> any blood
>> test for a hormone level) should be drawn in a red top tube...not
>> the red &
>> grey with the serum separator in it. The red and grey top tubes
>> can cause a
>> lower than correct level.....Interesting!!
>>
>> Cyndi
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