Following links may be of interest: #1. https://www.invitrogen.com #2. http://www.merckbiosciences.co.uk/ BLOT-Fast Stain™ Reagent *Cat. No. WB56 * Western Blotting Detection Systems & Standards<http://www.merckbiosciences.co.uk/Products/BrowseProductsByCategory.asp?catid=106>» Reagents for Blocking, Staining, and Stripping<http://www.merckbiosciences.co.uk/Products/BrowseProductsByCategory.asp?catid=1143> *A rapid, convenient method for visualizing protein transfer to nitrocellulose and PVDF membranes.* Formulation: 125 ml Reagent A (Fixer) and 125 ml Reagent B (Developer) Kit Contents: Reagent A (fixer) and Reagent B (developer) Comments: Stains as little as 0.5 ng of protein per band. Background remains unstained, leading to exceptional band visibility. Stain can be easily removed with warm water, and Western blotting can proceed with no changes in protocol. Kit includes fixer reagent and developer sufficient to stain 25 12 X 12 cm2 blots. Ipek Bosgelmez On 12/25/06, Mavi Gozler <mavigozler@xxxxxxxxx> wrote:
Perhaps all if not most of you are familiar with reversible staining of proteins electroblotted onto membranes (PVDF, nitrocellulose) to be immunochemically illuminated (westerns). Ponceau S has been around a long time and still seems reliable. BDH Laboratory Supplies (Poole UK) appears to offer something called "Instaview Nitrocellulose." The kit includes a concentrate of stain (Coomassie, I think), "enhancer" (which actually improves contrast by removing noise [background staining]), and a destain. I could not find a home page for this manufacturer. Perhaps they sell through vendors (B2B). I am also curious about the way scientists are illuminating their westerns typically. Do people use enzyme-conjugated secondary antibodies to produce visible colored precipitates or do they prefer chemiluminescent (and if so, how are you imaging them: film or digicam?) SMH __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com