[mti] universal primers

  • From: "David Brown" <brownds@xxxxxxxxxxxxx>
  • To: <mti@xxxxxxxxxxxxx>
  • Date: Tue, 24 Apr 2007 13:57:25 +0100

I was wondering whether anybody knew of any good universal invertebrate mtDNA 
primers. They do not have to be invertebrate-specific, but I need them to 
amplify most inverts, including worm and slug, with an amplicon size of 
100-300bp. I've tried various combinations of the Simon et al (1994) primers 
(C1J2183, C1J2195, C2J3400, C2J3571, C1N2329, C1N2353, C2N3494, C2N3661) but 
haven't found any which will amplify both worm and slug. Any suggestions 
welcome.

Regards
David Brown
Cardiff University

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