Dear All, In some earlier posts, getting singlets as fasta file had been discussed. Although i followed the recommendation, i can see no fasta file for singlets.Hhere is the command i used.. mira -project=project_p_run4 -fasta -job=denovo,genome,accurate,454 -highlyrepetitive -GE:not=8 -AS:sep=1 -SK:not=8 -SK:bph=18 -SK:mchr=4096 454_SETTINGS -ED:ace=1 -OUT:sssip=on -OUT:stsip=on so i can not see any information about singlets, else of txt files. i guess i miss a big notch, Thank you in advance.. Best, Visam