[mira_talk] sff_extract question
- From: Jeremy Volkening <volkening@xxxxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Mon, 03 Jan 2011 12:57:38 -0600
Hi,
When I ran sff_extract on my latest data files in preparation for input
into mira, the 5' cutoffs in the traceinfo xml file were off by one
basepair (these are MID-tagged reads). I imagine this is set by the
sequencing lab at the time of the run, but the strange thing is that the
cutoffs in the fasta files themselves (shown as lowercase characters)
are correct.
It's easy enough to do a global replace in the xml file, but I thought I
should mention it. I tried to look into the code to see what was going
on, by I don't know much python. Is this a bug or an incorrect setting
by the sequencing lab?
Jeremy
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