[mira_talk] mira an assembly combiner?
- From: Steven Robbens <steven.robbens@xxxxxxxxx>
- To: "mira_talk@xxxxxxxxxxxxx" <mira_talk@xxxxxxxxxxxxx>
- Date: Tue, 7 May 2013 06:35:11 +0000
Dear all,
I've done a targeted resequencing project of X targets in 20 different samples.
Per target and per sample, I performed first a mapping of all reads against the
target and second a de novo assembly of the mapped reads, using mira. No I want
(per target) to combine the 20 de novo assemblies into one, thereby creating a
kind of reference assembly.
My question: can I use mira to perform this second round of assembly, based on
an existing assembly rather than using individual reads? Can this be seen as a
kind of hybrid assembly? Or should I use tools like CAP3?
I'm currently using the development version of mira (3.9.15)
Thanks for the feedback,
Steven
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