On Wednesday 18 February 2009 Sven Klages wrote: > The Roche software itself is writing 5' and 3' trim points into the sff > file; the 5' trim point is usually set to base position 5 (after the key > sequence 'TCAG'). The software can (sfftools, sfffile) split sff > archives by their MID sequences, which means for every MID found > there is a new sff file created with the 5' trim point shifted by > 10 bases (or whatever length of MID is configured) to 3'. > [...] > just my 2p, Most valuable 2p you have there Sven. I don't have a MID data set myself, would you care to give a short "demo" on how to split the data sets with the Roche software? I'd immediately integrate this into the walk-through for working with 454 data. Regards, Bastien -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html