[mira_talk] Re: mid-tags

  • From: Bastien Chevreux <bach@xxxxxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Thu, 19 Feb 2009 01:11:04 +0100

On Wednesday 18 February 2009 Sven Klages wrote:
> The Roche software itself is writing 5' and 3' trim points into the sff
> file; the 5' trim point is usually set to base position 5 (after the key
> sequence 'TCAG'). The software can (sfftools, sfffile) split sff
> archives by their MID sequences, which means for every MID found
> there is a new sff file created with the 5' trim point shifted by
> 10 bases (or whatever length of MID is configured) to 3'.
> [...]
> just my 2p,

Most valuable 2p you have there Sven.

I don't have a MID data set myself, would you care to give a short "demo" on 
how to split the data sets with the Roche software? I'd immediately integrate 
this into the walk-through for working with 454 data.

Regards,
  Bastien

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