[mira_talk] Re: medium error: Very High Average Coverage

  • From: Dietmar Fernandez <dietmar.fernandez@xxxxxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Fri, 29 May 2015 12:41:54 +0200

That sounds OK for me. Good luck with your analysis.

Dietmar

2015-05-29 10:54 GMT+02:00 Rameez Mj <rameez03online@xxxxxxxxx>:

This is a follow up to my previous post "medium error: Very High Average
Coverage" on 28/5/2015.


Thanking Dietmar Fernandez and Felipe Lira for suggestion. I analysed
quality of my sample using fastqc and prinseq as suggested. I am
attaching the results here with. I am trying to filter my sample using
PRINSEQ. If I filter out all the exact duplicates and trim reads after a
length of 200bp will it be enough. Please mention suggestions if any.





--

Dietmar Fernández, Ph. D


*Bioinformatics Postdoctoral Fellow*


*Leading research at **IS*
*Global*Instituto de Salud Global de Barcelona

Hospital Clínic - Universitat de Barcelona


Centre Esther Koplowitz (CEK)

Carrer Rosselló 149.153, 1ª Planta

08036 Barcelona, Spain

Tel. +34 93 227 5707 (ext 4569)

dietmar.fernandez@xxxxxxxxxxxx <dietmar.fernandez@xxxxxxxxxx>

*www.isglobal.org <http://www.cresib.cat/>*

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