[mira_talk] how to interpret MIRA result
- From: Yunfei Li <yunfei8685@xxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Fri, 23 Mar 2012 19:45:46 -0400
Hi,
I am working on assembly ecoli genome by MIRA with a backbone. Some part of
the info report I am not quite understand, if someone can share some of his
experience? I am very new in this area, so any explanations would be
greatly helpful!
1. most importantly, by default setting, I only get one huge contig which
is almost same size as the backbone. My understanding is the assembly
process with backbone in MIRA is like mapping reads back, but what about
genome location without reads coverage? Besides, For de novo assembly we
usually pick the one with more and longer contigs as best assembly, and
here how can I evaluate it?
2. My data is from 454, but why "Max coverage per sequencing technology"
shows there are some Sanger data
--
Best,
Yunfei Li
--------------------------------------------------------------------------------------
Bioinformatics Specialist
Pugh Lab, Huck Institutes of the Life Science
Bioinformatics Consulting Center
Wartik Lab 502
Pennsylvania State University
State College, PA, 16803
Phone: 814-441-2383
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