On May 27, 2013, at 12:15 , Austen Chen <austen.chen@xxxxxxxxxxxxxxx> wrote: > The version I used was v3.4.1.1 Do yourself a favour and take the latest development version for Ion data: http://sourceforge.net/projects/mira-assembler/files/MIRA/development/ Docs in the package or online here: http://mira-assembler.sourceforge.net/docs-dev/DefinitiveGuideToMIRA.html Please read up the four or five sections on how to write manifest files http://mira-assembler.sourceforge.net/docs-dev/DefinitiveGuideToMIRA.html#sect_ref_config and here's a simple example for a mapping assembly with Illumina data (you'll be able to find out how to change file formats and technology used, I'm sure): http://mira-assembler.sourceforge.net/docs-dev/DefinitiveGuideToMIRA.html#sect_sxa_ma_writing_a_simple_manifest_file B.