[mira_talk] Re: extremely high illumina coverage
- From: Ganga Jeena <ganga.jeena@xxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Thu, 20 Oct 2011 18:31:50 +0200
If someone can tell me how to do away with this GC bias in reads
or a solution for it.
Regards,
Ganga Jeena
On Thu, Oct 20, 2011 at 2:09 PM, Phillip San Miguel <pmiguel@xxxxxxxxxx>wrote:
> On 10/18/2011 5:20 PM, Bastien Chevreux wrote:
>
>> On Oct 18, 2011, at 22:27 , Nestor Zaburannyi wrote:
>>
>>
>> GC rich data make the appearance of the GGCxG motif related sequencing
>> error more frequent. There is less sequencing data per se present and it has
>> more errors ... this combined with clipping may lead to zero assembly
>> coverage, even at higher raw coverages.
>>
>> B.
>>
>
> Does anyone know whether GGCxG is still and issue with v3 Illumina
> chemistry? How about with no-enrichment-PCR libraries?
>
> Phillip
>
>
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