Dear all, I have tried to use convert_project in order to get rid of the chimeric reads at the ends of the contigs, which prevent further contig-joining. I use: convert_project -f maf -t fasta -r c -v 2 infile.maf outfile.fasta but the contigs remain the same, I still have stretches on the ends with coverage = 1. Why these are not converted to N's? Can MIRA filter out regions with coverage=n during assembly? Any trick/switch? Sincerely yours Nestor -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html