On Apr 23, 2011, at 23:49 , Stephanie Pearl wrote: > I would like to convert_project to convert my fasta files into a fastq file, > but I'm not sure what my input should be. I saw the examples on how to > convert one file into another file type, but I would like to combine my > separate .fna and .qual files (in fasta format) into a single fastq file. Any > suggestions on this? How convenient that I just started writing a man page for convert_project. Back to your problem. convert_project does not have a mode to convert multiple files at once ... or to append to existing files (but maybe I should think of that). The only way to do what you want is to successively convert and then concatenate. As in: convert_project -f fasta -t fastq in1.fasta out1 convert_project -f fasta -t fastq in2.fasta out2 convert_project -f fasta -t fastq in3.fasta out3 ... cat out1.fastq out2.fastq out3.fastq ... >mynewfile.fastq Agreed, not the most effective way to handle things like these, but it works good enough most of the time. B. -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html