On Donnerstag 23 September 2010 Shabhonam Caim (TGAC) wrote: > Assembly with 1 Lane of Illumina ~32 million reads > About 1.5 million 454 reads Hello Shab, I fear that this will be one of the projects where MIRA has to capitulate. You are operating at the border of what MIRA can theoretically handle ... and crossed it. > Expected genome size ~150 Mb > Having to write log files to NFS drive That is ... bad. > I did try the miramem and I got the memory estimate around 80Gb, but its > utilising over 200Gb of memory. Can you please send me the whole log file? I'd like to know where the memory goes. > Here is the latest error message from MIRA. > [...] > "More than 2^31 skim hits would be taken ... this was not foreseen yet. > Please contact the author." That's the boundary the project crosses. I'm working on something to alleviate this problem, but it will take some time before I can roll that out, I first have a bug fix version on my list. Best, Bastien -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html