Hi all, I was doing a mira denovo assembly for solexa assembly and after 3 days of running , I just got an error message while it was searching for overlaps. Here I attached the error message and is anybody have any idea about it? The parameter i used is *mira --projrct=illumina_test --jobs=denovo,genome,accurate,solexa -DI:trt=/tmp/ -SK:not=30 SOLEXA_SETTINGS -GE:tismin=250:tismax=250 *and *the input data is a pair end reads based on the specified /1 and /2 naming scheme. *any help would be appreciated. Thanks, Robel* * On Wed, Aug 17, 2011 at 5:17 PM, Bastien Chevreux <bach@xxxxxxxxxxxx> wrote: > ** > > On Wednesday 17 August 2011 23:03:09 Lionel Guy wrote: > > > > You could either > > > > a) switch off all clipping routines in MIRA, i.e. > > > > > > > > "--noclipping -CL:pec:no" > > > > > > Well, I'll try that. But then my closing reads might go wrong, right? I > > > could then hard clip them before (they are soft clipped in the xml, but I > > > don't know if -noclipping -CL:pec:no overrides that) > > > MIRA will honour the clips in the XML on loading, so they're fine. > > > B. > > >
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mira_error.bmp
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