[mira_talk] Re: SNP detection for genomic sequence
- From: Bastien Chevreux <bach@xxxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Wed, 7 Jul 2010 23:46:08 +0200
On Mittwoch 07 Juli 2010 Raj Kumar, Praveen Kumar Mr. wrote:
> Hi,
> I have couple of questions here. First of all lemme say what I try to
> achieve. I am trying to assemble the EST's.
>
> Qn 1. Can I use multiple fasta files and accordingly multiple qual files.
> Qn 2. The ESTs I have is sequenced by two different techniques. To be
> precise, 6 million by 454 and 1 million by sanger. So I want to use both
> in one run. Is it possible? If so please guide me.
I'll answer both questions in one go: at the moment, you can use exactly one
FASTA (and FASTA quality) file per sequencing technology.
In your case
mira --project=... --job=est,denovo,accurate,sanger,454
would load one file for Sanger data and one file for 454 data.
B.
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