On Donnerstag 01 Juli 2010 Lionel Guy wrote: > I'm using mira 3.1.15 to assemble de novo a small (1.6 Mb) bacterial > genome, with an acceptable level of repeats (had 1-2 megahubs). I'm > using paired-end and non-paired-end 454 reads (300 kreads) and some long > Sanger reads (separate fosmid assemblies, 5-20kb long). > > I've already been running a very similar assembly (but with less Sanger > reads) earlier with a previous version of mira (3.0.4, if I remember > correctly), and had no particular problems. > > Now I'm running it again and got three times out of memory troubles, at > different points of the assembly, and running it on different machines > (twice on a local machine with 8Gb, and once on a cluster node with 24 > Gb). Ehm ... out of memory with 300k reads? This should not really happen. Could I have a look at that complete data set? B. -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html