Thank You very much for your answer.I launched the mapping yesterday on a server with 8*24GB and I have no error yet. I thought Mira was only using 4GB of the 32, that's why I did not try that earlier.
Anyway I will let you know if the mapping go through. Thanks again Gautier Le 17/08/2010 16:28, Bastien Chevreux a écrit :
----- original message --------I now use Mira since 3 months with 454 data. 1 month ago, I received Solexa data (around 29M reads). I want to use these solexa reads to correct/extend my 454 contigs. I have an Out of Memory error: [...] But the strange thing is that if I check, Mira only used 4GB of Memory on 24GB availableNope. Your system has 36 GiB. After loading the 29m reads, MIRA already used 32 GiB, 1 GiB was used by the OS and 3 GiB were free.What am I doing wrong?Your machine has a bad set-up: only 4 GiB swap for 36 GiB RAM is not ideal. I'd go for a 1:1 setup there. Furthermore, you are operating at the limit of your machine there: miramem will tell you that you need around 36 to 40 GiB RAM for that project as minimum. Well, looks like you need a bit more than you currently have as RAM + swap. Tip: try mapping 20m reads to see whether you like what MIRA is producing with your data. B.
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