On Tuesday 24 May 2011 17:42:21 Juan Daniel Montenegro Cabrera wrote: > I recently tried to assemble a 454 sequencing project. I have ~190 000 > reads of 236 bp average length. Although is not a highly demanding > assembly, the server i am using crashes all the time. It is pretty old and > has a lot of users. Do you know of any other free server i could use to > run the assembly? First of all: is this a real crash (a program error which results in such a fatal status that the process gets killed by the OS) ... or is it that MIRA just stumbles across something and exits cleanly, giving you a more or less clear explanation while doing so in the output log? The later is NOT a crash :-) Then ... if aut of your 190k read you have 180k which are the same or almost excatly the same, then this is for sure a very taxing assembly ... the number of reads not always tells the whole story. B.