[mira_talk] Re: Mira results much worse than newbler

  • From: Robin Kramer <kodream@xxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Thu, 14 Jul 2011 14:38:39 -0600

sff_extract trims of the first however many bases, in the least
sophisticated way.
sffinfo finds the adapters.

There is a big difference in the resultant assembly.  sffinfo still isn't
perfect though.

Sincerely yours,

Robin

On Thu, Jul 14, 2011 at 2:30 PM, Bastien Chevreux <bach@xxxxxxxxxxxx> wrote:

> On Jul 14, 2011, at 21:32 , Peter Cock wrote:
> > On Thursday, July 14, 2011, Robin Kramer <kodream@xxxxxxxxx> wrote:
> >> You should really use sffinfo, sff_extract doesn't clean the sequences.
> > What do you mean? Both Roche sffinfo and sff_extract can give you
> > the trimmed or untrimmed sequences as defined in the SFF file.
>
> Exactly the question which came to my mind.
>
> Robin?
>
> B.
>
>
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