On Thu, Dec 3, 2009 at 7:19 PM, ankit gupta <ankitgupta.iitg@xxxxxxxxx> wrote: > Hi, > > I am trying to use mira for a de novo assembly of a transcriptome but after > running for a few minutes, it gives me a memory related error. > > ... > Ouch, out of memory detected. > There is one line in the log file that caught my eye: Compiled in 32 bit mode This would suggest you are going to be limited to just 4GB of RAM. I think you need to recompile in 64bit mode. What OS are you using? I have a similar issue on Mac OS X 10.5 Leopard: //www.freelists.org/post/mira_talk/MIRA3r4-and-memory-on-Mac-OS-X Peter -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html