On Freitag 23 Juli 2010 Saulo Alves wrote: > Here follows, > Thank you very much Hmmm. That screenshot looks OK to me. I see no rail read which is not covered by any normal Solexa read. Generally speaking, areas of a reference genome (or rail reads) not covered by resequenced reads mean that you most probably have a deletion in your sequenced genome. If your sequencing sample was a mixed population (with and without deletion), then you may notice a sharp drop in coverage. But then again ... I wonder why you see rail read. Did you take an intermediate result from the log directory? Regards, Bastien -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html