[mira_talk] Re: Mappging to Reference

  • From: Saulo Alves <sauloal@xxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Fri, 23 Jul 2010 21:20:53 +0200

Here follows,
Thank you very much

----------------
s.


On Fri, Jul 23, 2010 at 9:10 PM, Bastien Chevreux <bach@xxxxxxxxxxxx> wrote:

> On Freitag 23 Juli 2010 Saulo Alves wrote:
> > I'm having problems with the rail reads.
> > Can you please explain to me their nature?
>
> Ummm ... where do you see rail reads? They're not put into the results ...
> are
> they?
>
> > I've noticed that a good deal of my sequences have only the rr covering
> it.
> >  eg
> >
> > ref      aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
> > read1 aaaaaaaaaa
> > read2                             aaaaaaaaaaaaaaaaa
> > rr1                aaaaaaaaaaaaaaa
> >
> > In this case, i was assuming that the region covered only by RR are
> > regions without sequencing information. Turns out, more than 80% of my
> > sequence is like that. Even worse, there are several SNP/GAP/INS/DEL
> > in those regions, what is a bit strange.
>
> Could you make a screenshot depicting this please? I'd like to see exactly
> what you mean.
>
> B.
>
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Attachment: rr.png
Description: PNG image

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