[mira_talk] Re: Making the best assembly
- From: Bastien Chevreux <bach@xxxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Tue, 8 Dec 2015 23:02:13 -0500
On 07 Dec 2015, at 12:07 , C Jenkins <cej.jenkins@xxxxxxxxx> wrote:
We are talking about cDNA/RNASeq data for a eukaryotic parasite.
There were 72276 reads used in the Illumina assembly, but a big chunk of my
raw reads were not used.
Okay … is there a reason you used only this amount of reads? I normally take 10
to 15m pairs (20 to 30m reads) for RNASeq assemblies to reconstruct an
acceptable transcriptome.
B.
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