Dear all,
MIRA V3 has been released at SourceForge.
http://sourceforge.net/projects/mira-assembler/
Documentation for MIRA can be found both in the binary packages and
on the
MIRA-Wiki, the latter being here:
http://sourceforge.net/apps/mediawiki/mira-assembler/
So, what's new in this release? Actually, a lot!
The 3.0 version of MIRA is the result of a long development to make
de-novo
and mapping assemblies of Sanger, 454 and Solexa (Illumina) data as
easy and
straightforward as possible while keeping a maximum accuracy.
Another focus was to make it possible to use results from Solexa
mapping
projects in current finishing programs, not only viewers. MIRA
introduces for
that the notion of coverage equivalent reads (CER) which reduces the
data
volume by 70 to 90%. This allows painless use of such data sets in
"gap4" and
"consed".
A third focus was to reduce time for researchers to find and evaluate
differences in mapping assemblies. MIRA supports that by setting
tags and
places of interest and creating easy to understand HTML files and
tables ready
to be imported into spreadsheet programs.
As a lot has changed since the 2.8.x series of MIRA, the following
list has
just a few highlights which came in during the 2.9.x series:
- sequencing technologies: MIRA handles different sequencing
technologies
independently from each other and has specialised routine for
working with
each of them.
- command-line parameters: MIRA has now a handful of "Do-What-I-
Mean" one-stop
switches which allows to configure the assembler for 90% of all use
cases. Furthermore, many parameters can be adjusted for each
sequencing
technology so that the assembly engine can be tweaked for very
specialised
cases if needed.
- all sequencing technologies (Sanger, 454, Solexa) have now
- recognition of chimeras
- new assembly routines to for improved repeat resolving
- improved data preprocessing that gets rid of low quality data and
sequencing errors at ends of reads ... even when no quality data is
available.
- 454 data:
- fully developed capability for de-novo and mapping assembly of
454 data
(paired and non-paired)
- automated contig editor to remove most obvious and/or annoying
sequencing
errors
- improved consensus calling streamlined to minimise the dreaded
homopolymer
problem
- Solexa data
- can handle Solexa data of any length, no restriction to very short
sequences.
- memory/space saving: MIRA has special mapping mode which creates
data
so that widely used finishing tools like gap4 and consed can load
these
projects and still be fairly quick
- alignments enriched with features: MIRA adds information like
repetitiveness
or repeat marker bases as tags in the assembly so that these can be
used
during finishing
- assembly information files: MIRA writes more information files
which can be
easily parsed and/or read
- mapping assemblies: MIRA has a full SNP analysis for prokaryotic
data
- comprehensive tables and HTML result files (mapping assembly): the
convert_project program can now create easy to use tables and HTML
files
which show the data in a way suited for less computer-interested
people
(biologists etc. :-)
- memory management: MIRA can now be told to use an upper limit of
RAM.
- file formats: MIRA can now parse or write more file formats.
Notable are
change from SSAHA to SSAHA2 for clipping, FASTQ for data input and
MAF
format for output.
- MacOS support: MIRA now compiles on MacOS-X
- speed and memory: compared to 2.8.x, MIRA now uses way less memory
and is a
lot faster.
- tons of other features, tweaks and bug fixes. See the
CHANGES_old.txt file
Have a lot of fun with MIRA :-)
Bastien
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