[mira_talk] Re: Hybrid EST assembling (Two species, A and B, Backbone from A, solexa sequences from B)

  • From: Bastien Chevreux <bach@xxxxxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Wed, 2 May 2012 21:32:55 +0200

On May 2, 2012, at 16:08 , Arnaud Ungaro wrote:
> In exemple, at  the last locus ,the majority of reads are T, but consensus 
> set to A (same as backbone).
> Maybe by setting backbone's quality lower ?

I'm not sure that this will help: if I recall correctly, I think I implemented 
a rule like "if you cannot decide between two or more bases for the consensus, 
then take the backbone base if there are reads supporting that decision".

Which is the case in the screenshot you've shown ("3823-HWI...").

B.



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