[mira_talk] Re: Hybrid EST assembling (Two species, A and B, Backbone from A, solexa sequences from B)

  • From: Arnaud Ungaro <arnaud.ungaro@xxxxxxxxxxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Wed, 2 May 2012 23:22:43 +0200

Ok, I'll look at this , and try your advices.
Many thanks

Arnaud.

2012/5/2 Bastien Chevreux <bach@xxxxxxxxxxxx>:
> On May 2, 2012, at 21:57 , Arnaud Ungaro wrote:
>> Thank you about this precision. It is possible to change this rule ?
>
> In principle yes, but only in the code and I presently do not have time for 
> this
>
>> Or I have to 'filter' and remove this kind of sequences ? (Like
>> "3823-HWI...")
>
> Hmmm .. maybe not filter out, but try setting the quality of those sequences 
> to "5" for all bases. I haven't tried it out, but in theory this should let 
> those sequences fly under the radar in so far as that when conflict occur 
> with other, regular reads, the regular reads will be preferred for building 
> the consensus.
>
> B.
>
>
>
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