Hi all, Can anyone tell me how much disk space is required for EST assembly of 3 GB fasta file and 8GB qual file. I am asking this because, mira run stopped for my last two times saying something like Disk full?. I have 250 GB limit in my cluster machine. When I started MIRA job I had only 20 GB occupied, but after 1 week of run I see all 250 GB has been utilized and still MIRA stopped. How much disk space I require? Below are the commands I used, mira --project=EACh --job=est,denovo,accurate,sanger,454 SANGER_SETTINGS -CL:qc=no 454_SETTINGS -LR:mxti=no >& log_assembly.txt My input files are named as described like EACh_in.sanger.fasta EACh_in.sanger.fasta.qual EACh_in.454.fasta EACh_in.454.fasta.qual regards, Praveen -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html