[mira_talk] Re: Bivalve EST assembling
- From: Juan Fernandez Tajes <jfernandezt@xxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Wed, 12 Oct 2011 16:14:05 +0200
Hi all,
I have launched mira with -job=denovo,est,accurate,454 -highlyrepetitive
-notraceinfo, nevertheless, mira stops and warns me about megahubs in my
read (approximately a 2%). The program and the manual suggest set
-SK:nrr set to 10,
Do you think that with these parameters will fix the problem:
-job=denovo,est,accurate,454 -highlyrepetitive -notraceinfo
-SK:mnr=yes:nrr=10
Many thanks for the help
Juan
El jue, 06-10-2011 a las 00:04 +0200, Bastien Chevreux escribió:
> On Monday 03 October 2011 23:47:58 Juan Fernandez Tajes wrote:
>
> > [...]
>
> > -job=denovo,est,accurate,454 -highlyrepetitive -notraceinfo, are
> these
>
> > parameters appropriate??
>
>
> If you're not looking for something special, yes.
>
>
> > It would be a better idea running a "draft", or "normal" assembly
> before an
>
> > "accurate" one?
>
>
> Not really.
>
>
> B.
>
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