On Friday 15 April 2011 15:18:45 Andrei Tudor wrote: > So there is no need to use a program like ABACAS to build a pseudomolecule? > Also after the checking through gap5 and if there is no need for a > pseudomolecule, can i submit it for annotation? Ahm ... what you got probably was a pure mapping assembly. If I were you, I would take all the reads from the debris file of that assembly and assemble these de-novo to hunt for genpome stretches which were not prsent in your reference genome. B.