[mira_talk] Assembly of NCBI Data

  • From: "Thomas, Dallas" <Dallas.Thomas@xxxxxxxxx>
  • To: <mira_talk@xxxxxxxxxxxxx>
  • Date: Fri, 7 Jan 2011 16:24:23 -0600

Hello One and All,

 

  Have 13,900 sequences comprised of NCBI nt, est and gss data.  This is
just the fast information obtained from NCBI.  Looking at doing a
de-novo assembly on the data, however am a bit baffled on the --joblist
and options I should be using.  Does one just use sanger? Or would one
assume that a high proportion of est, etc might come from 454?  Would
you use "est" in the joblist for all of the sequences or assembly only
the est using "est" and use genome for the gss and nt.

 

  Any good strategies one might recommend?

 

Thanks,

Dallas

Other related posts: