Hello One and All, Have 13,900 sequences comprised of NCBI nt, est and gss data. This is just the fast information obtained from NCBI. Looking at doing a de-novo assembly on the data, however am a bit baffled on the --joblist and options I should be using. Does one just use sanger? Or would one assume that a high proportion of est, etc might come from 454? Would you use "est" in the joblist for all of the sequences or assembly only the est using "est" and use genome for the gss and nt. Any good strategies one might recommend? Thanks, Dallas